Genotyping and primer details


Genotyping and primer details for 276A02 - At3g57520
Line ID 276A02
Locus At3g57520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21291653 of line 276A02 Chr3:21291653
   
Insertion allele amplicon 8474 - MK33
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MK33: 5'-GAACCAATCCCTTTGAAGTTATCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21291993
   
wt amplicon (over InsPos) 37dy - MK33
wt amplicon start Chr3:21291230
wt amplicon end Chr3:21291993
wt amplicon size 764 bp
Locus specific primer MK33: 5'-GAACCAATCCCTTTGAAGTTATCA-3'
2nd locus specific primer 37dy: 5'-ACTAAGGATTCGACAATTCCAATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11