Genotyping and primer details


Genotyping and primer details for 308D07 - At1g49780
Line ID 308D07
Locus At1g49780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18430031 of line 308D07 Chr1:18430031
   
Insertion allele amplicon 8474 - BE54
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BE54: 5'-CCCTAATTTCAATCGAAGAATCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18429647
   
wt amplicon (over InsPos) 41dy - BE54
wt amplicon start Chr1:18429647
wt amplicon end Chr1:18430341
wt amplicon size 695 bp
Locus specific primer BE54: 5'-CCCTAATTTCAATCGAAGAATCAA-3'
2nd locus specific primer 41dy: 5'-TCAATTCCAAATTCTTCAAAGCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11