Genotyping and primer details


Genotyping and primer details for 311D06 - At2g35330
Line ID 311D06
Locus At2g35330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14869711 of line 311D06 Chr2:14869711
   
Insertion allele amplicon 8474 - JL29
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JL29: 5'-GAAACATTCTTCACTTCCGAAGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14870288
   
Insertion allele amplicon 8474 - JL30
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JL30: 5'-ATCATTTTGCCATCAATTAAAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14869105
   
wt amplicon (over InsPos) JL30 - JL29
wt amplicon start Chr2:14869105
wt amplicon end Chr2:14870288
wt amplicon size 1184 bp
Locus specific primer JL29: 5'-GAAACATTCTTCACTTCCGAAGAC-3'
2nd locus specific primer JL30: 5'-ATCATTTTGCCATCAATTAAAACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11