Genotyping and primer details


Genotyping and primer details for 372E03 - At5g60740
Line ID 372E03
Locus At5g60740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24426547 of line 372E03 Chr5:24426547
   
Insertion allele amplicon 8474 - 11em
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11em: 5'-AGTCCGAGTCTTTGGTTCTATCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24426280
   
Insertion allele amplicon 8474 - MD12
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MD12: 5'-AGTTCAGGTGACTTATCCGAAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24426992
   
wt amplicon (over InsPos) 11em - MD12
wt amplicon start Chr5:24426280
wt amplicon end Chr5:24426992
wt amplicon size 713 bp
Locus specific primer MD12: 5'-AGTTCAGGTGACTTATCCGAAAGA-3'
2nd locus specific primer 11em: 5'-AGTCCGAGTCTTTGGTTCTATCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11