Genotyping and primer details


Genotyping and primer details for 376D01 - At4g10380
Line ID 376D01
Locus At4g10380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:6433460 of line 376D01 Chr4:6433460
   
Insertion allele amplicon 8474 - AW03
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AW03: 5'-AACGAGCATCAACCAATCATAATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:6432842
   
wt amplicon (over InsPos) 01ua - AW03
wt amplicon start Chr4:6432842
wt amplicon end Chr4:6433990
wt amplicon size 1149 bp
Locus specific primer AW03: 5'-AACGAGCATCAACCAATCATAATA-3'
2nd locus specific primer 01ua: 5'-TAGGAACCCTACGTAAGCCTAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11