Genotyping and primer details


Genotyping and primer details for 396D09 - At4g02420
Line ID 396D09
Locus At4g02420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1064441 of line 396D09 Chr4:1064441
   
Insertion allele amplicon 8474 - EQ43
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ43: 5'-ATTACATGCCCAATGGAAGTTTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1065099
   
Insertion allele amplicon 8474 - KF54
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KF54: 5'-ATTGGTCCTCCAATGATATAGTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:9811975
   
wt amplicon (over InsPos) KF54 - EQ43
wt amplicon start Chr4:1065099
wt amplicon end Chr1:9811975
wt amplicon size 1083 bp
Locus specific primer EQ43: 5'-ATTACATGCCCAATGGAAGTTTAG-3'
2nd locus specific primer KF54: 5'-ATTGGTCCTCCAATGATATAGTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11