Genotyping and primer details


Genotyping and primer details for 402A07 - At4g26400
Line ID 402A07
Locus At4g26400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13345595 of line 402A07 Chr4:13345595
   
Insertion allele amplicon 8474 - KP42
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KP42: 5'-CACAAATGGTGAATCCAGTTATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13345992
   
wt amplicon (over InsPos) 02tx - KP42
wt amplicon start Chr4:13345121
wt amplicon end Chr4:13345992
wt amplicon size 872 bp
Locus specific primer KP42: 5'-CACAAATGGTGAATCCAGTTATTG-3'
2nd locus specific primer 02tx: 5'-TCTTGTTCTCGGTCTTACAGGATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11