Genotyping and primer details


Genotyping and primer details for 410B07 - At5g07620
Line ID 410B07
Locus At5g07620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2408130 of line 410B07 Chr5:2408130
   
Insertion allele amplicon 8474 - EQ53
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ53: 5'-AGATAGAACTCATCATCGCCAGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2407606
   
wt amplicon (over InsPos) CN29 - EQ53
wt amplicon start Chr5:2407606
wt amplicon end Chr5:2408751
wt amplicon size 1146 bp
Locus specific primer EQ53: 5'-AGATAGAACTCATCATCGCCAGTT-3'
2nd locus specific primer CN29: 5'-TTTCTTAGACCGGTGTGTACAGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11