Genotyping and primer details


Genotyping and primer details for 413C02 - At4g16070
Line ID 413C02
Locus At4g16070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9098214 of line 413C02 Chr4:9098214
   
Insertion allele amplicon 8474 - 22uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 22uj: 5'-TGAGAATTGCACTGTTGAAATAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9098835
   
wt amplicon (over InsPos) 01uu - 22uj
wt amplicon start Chr4:9098173
wt amplicon end Chr4:9098835
wt amplicon size 663 bp
Locus specific primer 22uj: 5'-TGAGAATTGCACTGTTGAAATAGG-3'
2nd locus specific primer 01uu: 5'-GAAAGACTGAATCATTTAGCAGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11