Genotyping and primer details


Genotyping and primer details for 414B10 - At1g72850
Line ID 414B10
Locus At1g72850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27414922 of line 414B10 Chr1:27414922
   
Insertion allele amplicon 8474 - 32lq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 32lq: 5'-AAGAAAATCTCAGATAAACCGCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27415470
   
wt amplicon (over InsPos) 03mc - 32lq
wt amplicon start Chr1:27414314
wt amplicon end Chr1:27415470
wt amplicon size 1157 bp
Locus specific primer 32lq: 5'-AAGAAAATCTCAGATAAACCGCAA-3'
2nd locus specific primer 03mc: 5'-CAGATGACAAGATTCAAGAATGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11