SimpleSearch - Line and FST details
Line specific information
| Line ID | 414B10 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N439670) |
| Segregation Analysis | 50:49:39 |
| Confirmed for Hit | At1g72850 At2g33710 |
| Parent of DUPLO pair | 2533 |
| Parent of pair(s) | none |
| Note |
Gene hit At1g72850
| Sequence (A. th genome BLAST matches underlined) | >74-K026070-022-414-B10-8409 GAAATCGAGGTTTTGTTGAAACCGACAAAAAACCTGTGTTTGAAATTGTTCATCAATCGG TTAGCAAGCTCTTTCCGTATGGGATCCTCCCTATAGGGAGAAGGATGANNAGNGNTGGGA GAAGGAACTGCNTCAACTGGAGGGAGANCAAGAAAAAGCTATTATGGAAATCACGAGCAA GAGATACACAAGAGCAGGAAAAAGGGAAGAAGATAACGAGAATTTCCCATCATTTATTCT CTTGAGNGATGATTAATAAGTGAATGAATCCCTCAAGAAGATATTAATAAAGTGATGATC TGTTA |
| GenBank Accession | CR397115 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:27414894 - go to primer design |
| BLAST e Value | 5e-76 |
| Hit Clone Code (BAC ID) | F3N23 |
| Hit Gene Code | At1g72850 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Disease resistance protein (TIR-NBS class) |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit At3g53810
| Sequence (A. th genome BLAST matches underlined) | >74-K018037-022-414-B10-8409 TTGATATATCCCAACACTAAGCGGCCATGGAATCGCCTTCGTTGTAGCTCCAACGCTATG GGATCCTCCCTATAGTGAGAGTTAAAATGAAACTACATTACGGGATTTTCCCTATAGCGA GACGGACATCTACCTTTTTGAATTGAAAAAAAATAGGAAATTACTCTTTCTTTTTCTCCA AATAGACCATCATACTCATTGCTGATCCATGAAAATATTTCTGCCATGAAGGAATTTTCA ATTGCAAATATCCT |
| GenBank Accession | BX288422 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:19934898 - go to primer design |
| BLAST e Value | 3e-20 |
| Hit Clone Code (BAC ID) | F5K20 |
| Hit Gene Code | At3g53810 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Concanavalin A-like lectin protein kinase family protein |
| Insertion Classification | CDSi |
| Confirmation Status | failed, line belongs to a contamination group, please check line 413B10 |
Gene hit At2g33710
| Sequence (A. th genome BLAST matches underlined) | >74-K018043-022-414-B10-8409 TATCCTGTTATTTCCCGGACATGAAGCCTTTACATTGAATTGCAAATGATAATATCTGTC TTATTATCCAATGCTCATATATTTTTCAACGCGTTGAATGATGAAATGGTTGGTAAATAA AAATGAAACTAAACTATGGGATCCTCCCTATAGTGAGACTGAGATCTACGCTTTTGAATT GAATACACTTTGATACTAACACTTTCTTTTTCTCTTTACTGACTCTGATACTGATTGCTG ATCCATGTAGATTTCCCGGACTCCCTGCCATTTACAATTGAATTGCAAATGATAATATCT GTCTTATTATCCAATGCTCATATATTTTTCAACGCGTTGAATGATGAAATGGGTGGTAAA TAAAAATGAAACTAAACTATGGGATCCTCCCTATAGTGAGGACCCGCGATAGTCGGGTAA GTGGACAACACGATTACCCCGCGGGGTTTTCAAGCAAGCCCCCACGACAGCTTCCAACTA CACCCTTCCTTCCTTTTTAGAACCCTCCATCTAAACAACAACTCACACCC |
| GenBank Accession | BX288423 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr2:14258723 - go to primer design |
| BLAST e Value | 1e-40 |
| Hit Clone Code (BAC ID) | T1B8 |
| Hit Gene Code | At2g33710 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Integrase-type DNA-binding superfamily protein |
| Insertion Classification | TS2TE (3') |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
|
Last Updated on 10.06.2021 13:37 |
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