Genotyping and primer details


Genotyping and primer details for 447C02 - At1g12640
Line ID 447C02
Locus At1g12640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4303814 of line 447C02 Chr1:4303814
   
Insertion allele amplicon 8474 - 43by
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 43by: 5'-ACTTCCAATGATGGAGGAACATAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4304151
   
wt amplicon (over InsPos) 22da - 43by
wt amplicon start Chr1:4303257
wt amplicon end Chr1:4304151
wt amplicon size 895 bp
Locus specific primer 43by: 5'-ACTTCCAATGATGGAGGAACATAC-3'
2nd locus specific primer 22da: 5'-TTTGTTAGTGGGGTACATTGATGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11