Genotyping and primer details


Genotyping and primer details for 454C04 - At4g34850
Line ID 454C04
Locus At4g34850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16609422 of line 454C04 Chr4:16609422
   
Insertion allele amplicon 3144 - FP75
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FP75: 5'-AGCCTCTCTTTGAGCTTCATACTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16609159
   
Insertion allele amplicon 8474 - JL06
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JL06: 5'-TCACATGTTGGTATGGTTTGTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16610035
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11