Genotyping and primer details


Genotyping and primer details for 463E06 - At5g45840
Line ID 463E06
Locus At5g45840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:18594841 of line 463E06 Chr5:18594841
   
Insertion allele amplicon 8474 - EQ75
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ75: 5'-GAAAAATCGAAAGGGTTTAAGGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:18595637
   
Insertion allele amplicon 8474 - KU47
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KU47: 5'-GACATTAGTCTCTGGTTCAGGAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:18594507
   
wt amplicon (over InsPos) KU47 - EQ75
wt amplicon start Chr5:18594507
wt amplicon end Chr5:18595637
wt amplicon size 1131 bp
Locus specific primer EQ75: 5'-GAAAAATCGAAAGGGTTTAAGGAC-3'
2nd locus specific primer KU47: 5'-GACATTAGTCTCTGGTTCAGGAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11