Genotyping and primer details


Genotyping and primer details for 495D06 - At4g16442
Line ID 495D06
Locus At4g16442 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9272215 of line 495D06 Chr4:9272215
   
Insertion allele amplicon 8474 - 06ov
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06ov: 5'-GGTCTCAAATTACCTAAAAGGGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9271685
   
wt amplicon (over InsPos) 04pc - 06ov
wt amplicon start Chr4:9271685
wt amplicon end Chr4:9272738
wt amplicon size 1054 bp
Locus specific primer 06ov: 5'-GGTCTCAAATTACCTAAAAGGGGT-3'
2nd locus specific primer 04pc: 5'-TTTTTGTTGTTATTGTTGGGATTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11