Genotyping and primer details


Genotyping and primer details for 532E11 - At4g23010
Line ID 532E11
Locus At4g23010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:12060575 of line 532E11 Chr4:12060575
   
Insertion allele amplicon 8474 - MP34
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MP34: 5'-TCTATTTTGTGTCTCCTTCATCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12060232
   
wt amplicon (over InsPos) 07dw - MP34
wt amplicon start Chr4:12060232
wt amplicon end Chr4:12061060
wt amplicon size 829 bp
Locus specific primer MP34: 5'-TCTATTTTGTGTCTCCTTCATCCA-3'
2nd locus specific primer 07dw: 5'-GAAAGTGACAAAGAGTGGAACCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11