Genotyping and primer details


Genotyping and primer details for 556E08 - At1g06680
Line ID 556E08
Locus At1g06680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2048987 of line 556E08 Chr1:2048987
   
Insertion allele amplicon 8474 - JV17
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JV17: 5'-CCTTTGGCATATGCTTCAATAGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2049584
   
Insertion allele amplicon 8474 - JV18
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JV18: 5'-GCAAAGAGATTGAGTATCCAGGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2048390
   
wt amplicon (over InsPos) JV18 - JV17
wt amplicon start Chr1:2048390
wt amplicon end Chr1:2049584
wt amplicon size 1195 bp
Locus specific primer JV17: 5'-CCTTTGGCATATGCTTCAATAGAT-3'
2nd locus specific primer JV18: 5'-GCAAAGAGATTGAGTATCCAGGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11