Genotyping and primer details


Genotyping and primer details for 585B07 - At5g49770
Line ID 585B07
Locus At5g49770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20226878 of line 585B07 Chr5:20226878
   
Insertion allele amplicon 8474 - EU08
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU08: 5'-TGATGGTGAAACAGGTCTACAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20226207
   
Insertion allele amplicon 3144 - NK74
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer NK74: 5'-ATCAATTATTGCGTTAGGGTTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20227280
   
wt amplicon (over InsPos) NK74 - EU08
wt amplicon start Chr5:20226207
wt amplicon end Chr5:20227280
wt amplicon size 1074 bp
Locus specific primer EU08: 5'-TGATGGTGAAACAGGTCTACAAAG-3'
2nd locus specific primer NK74: 5'-ATCAATTATTGCGTTAGGGTTTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11