Genotyping and primer details


Genotyping and primer details for 585H10 - At5g58150
Line ID 585H10
Locus At5g58150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23531757 of line 585H10 Chr5:23531757
   
Insertion allele amplicon 8474 - 31oz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 31oz: 5'-GGAGTTGGGTTCTGTGTTGTTTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23531131
   
wt amplicon (over InsPos) 02ps - 31oz
wt amplicon start Chr5:23531131
wt amplicon end Chr5:23532159
wt amplicon size 1029 bp
Locus specific primer 31oz: 5'-GGAGTTGGGTTCTGTGTTGTTTAT-3'
2nd locus specific primer 02ps: 5'-CTTCACACCCTTGATCTTTCTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11