Genotyping and primer details


Genotyping and primer details for 608F05 - At5g14940
Line ID 608F05
Locus At5g14940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:4833133 of line 608F05 Chr5:4833133
   
Insertion allele amplicon 8474 - 07up
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07up: 5'-GAAATGGTTTAGCTTTGAGGTCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4832818
   
wt amplicon (over InsPos) 02vj - 07up
wt amplicon start Chr5:4832818
wt amplicon end Chr5:4833562
wt amplicon size 745 bp
Locus specific primer 07up: 5'-GAAATGGTTTAGCTTTGAGGTCTG-3'
2nd locus specific primer 02vj: 5'-TCCATAATGTGATATTGGGGATCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11