Genotyping and primer details


Genotyping and primer details for 635C09 - At1g72370
Line ID 635C09
Locus At1g72370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27242997 of line 635C09 Chr1:27242997
   
Insertion allele amplicon 8474 - 08hd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08hd: 5'-TTTACAGGGAACCTGAGGAGACTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27243501
   
wt amplicon (over InsPos) 09ig - 08hd
wt amplicon start Chr1:27242481
wt amplicon end Chr1:27243501
wt amplicon size 1021 bp
Locus specific primer 08hd: 5'-TTTACAGGGAACCTGAGGAGACTA-3'
2nd locus specific primer 09ig: 5'-GCGCCTAGTAGTAGCTGTCTTGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11