Genotyping and primer details


Genotyping and primer details for 645B10 - At1g13450
Line ID 645B10
Locus At1g13450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4613100 of line 645B10 Chr1:4613100
   
Insertion allele amplicon 8474 - 35qz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 35qz: 5'-CAGATCTGCTTTTGGGTTAAGAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4613404
   
wt amplicon (over InsPos) 05rt - 35qz
wt amplicon start Chr1:4612722
wt amplicon end Chr1:4613404
wt amplicon size 683 bp
Locus specific primer 35qz: 5'-CAGATCTGCTTTTGGGTTAAGAAC-3'
2nd locus specific primer 05rt: 5'-TGACTCTGATACCACACACAAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11