Genotyping and primer details


Genotyping and primer details for 740A01 - At2g30820
Line ID 740A01
Locus At2g30820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13130936 of line 740A01 Chr2:13130936
   
Insertion allele amplicon 8474 - CR69
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CR69: 5'-TGTTTGCTTGAGACATGTTCTTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13130326
   
wt amplicon (over InsPos) 09ua - CR69
wt amplicon start Chr2:13130326
wt amplicon end Chr2:13131282
wt amplicon size 957 bp
Locus specific primer CR69: 5'-TGTTTGCTTGAGACATGTTCTTCT-3'
2nd locus specific primer 09ua: 5'-AAAGAGTAAATTCCAGCTCCATGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11