Genotyping and primer details


Genotyping and primer details for 752H06 - At2g26490
Line ID 752H06
Locus At2g26490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11269382 of line 752H06 Chr2:11269382
   
Insertion allele amplicon 8474 - 13ea
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13ea: 5'-ACCAACAAGGAAAATACACAAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11268864
   
wt amplicon (over InsPos) 07es - 13ea
wt amplicon start Chr2:11268864
wt amplicon end Chr2:11269888
wt amplicon size 1025 bp
Locus specific primer 13ea: 5'-ACCAACAAGGAAAATACACAAAGC-3'
2nd locus specific primer 07es: 5'-GTGTTTATTGATGCCGCTTAATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11