Genotyping and primer details


Genotyping and primer details for 761H03 - At3g14960
Line ID 761H03
Locus At3g14960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5037533 of line 761H03 Chr3:5037533
   
Insertion allele amplicon 8474 - 36fm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 36fm: 5'-CAAAGACCATGAATTAGAGCCTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5037208
   
wt amplicon (over InsPos) 06gf - 36fm
wt amplicon start Chr3:5037208
wt amplicon end Chr3:5037966
wt amplicon size 759 bp
Locus specific primer 36fm: 5'-CAAAGACCATGAATTAGAGCCTTC-3'
2nd locus specific primer 06gf: 5'-CGTCGTTACAAAATCATGTCATCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11