Genotyping and primer details


Genotyping and primer details for 787F05 - At2g17310
Line ID 787F05
Locus At2g17310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:7531226 of line 787F05 Chr2:7531226
   
Insertion allele amplicon 8474 - 36dl
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 36dl: 5'-GTGATATTCCCCAAATTTTTCCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:7530833
   
wt amplicon (over InsPos) 04jf - 36dl
wt amplicon start Chr2:7530833
wt amplicon end Chr2:7531669
wt amplicon size 837 bp
Locus specific primer 36dl: 5'-GTGATATTCCCCAAATTTTTCCTT-3'
2nd locus specific primer 04jf: 5'-AAGATCTTGTAAACCTTTTCGGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11