Genotyping and primer details


Genotyping and primer details for 791B01 - At2g39790
Line ID 791B01
Locus At2g39790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16595653 of line 791B01 Chr2:16595653
   
Insertion allele amplicon 8474 - CD96
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CD96: 5'-ACTATTTTGCAAACACACCCATTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16596247
   
wt amplicon (over InsPos) CD97 - CD96
wt amplicon start Chr2:16595202
wt amplicon end Chr2:16596247
wt amplicon size 1046 bp
Locus specific primer CD96: 5'-ACTATTTTGCAAACACACCCATTT-3'
2nd locus specific primer CD97: 5'-CTGACATTCTATTGGGGTTTGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11