Genotyping and primer details


Genotyping and primer details for 791G02 - At3g49670
Line ID 791G02
Locus At3g49670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18417656 of line 791G02 Chr3:18417656
   
Insertion allele amplicon 8474 - EU68
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU68: 5'-AAATCTCCGGTTAAATTGTTGTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18418213
   
wt amplicon (over InsPos) BH09 - EU68
wt amplicon start Chr3:18417464
wt amplicon end Chr3:18418213
wt amplicon size 750 bp
Locus specific primer EU68: 5'-AAATCTCCGGTTAAATTGTTGTTG-3'
2nd locus specific primer BH09: 5'-TCCACCACATGCATATGTTTCTAT-3'
   
wt amplicon (over InsPos) 01lz - EU68
wt amplicon start Chr3:18417201
wt amplicon end Chr3:18418213
wt amplicon size 1013 bp
Locus specific primer EU68: 5'-AAATCTCCGGTTAAATTGTTGTTG-3'
2nd locus specific primer 01lz: 5'-GTCGAATTATAACATCAGGACCGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11