Genotyping and primer details


Genotyping and primer details for 800G12 - At5g37840
Line ID 800G12
Locus At5g37840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:15062796 of line 800G12 Chr5:15062796
   
Insertion allele amplicon 8474 - 01db
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01db: 5'-GGGAACACTATAATGGTGAGGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:15063324
   
wt amplicon (over InsPos) 09em - 01db
wt amplicon start Chr5:15062240
wt amplicon end Chr5:15063324
wt amplicon size 1085 bp
Locus specific primer 01db: 5'-GGGAACACTATAATGGTGAGGAGA-3'
2nd locus specific primer 09em: 5'-ATGCTTCTGCATTTTATTTGGAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11