Genotyping and primer details


Genotyping and primer details for 876F12 - At1g57610
Line ID 876F12
Locus At1g57610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:21337615 of line 876F12 Chr1:21337615
   
Insertion allele amplicon 8474 - 32uv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 32uv: 5'-TGATGATGCTGGTGTTATTTTGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:21338101
   
wt amplicon (over InsPos) 03vj - 32uv
wt amplicon start Chr1:21337375
wt amplicon end Chr1:21338101
wt amplicon size 727 bp
Locus specific primer 32uv: 5'-TGATGATGCTGGTGTTATTTTGAT-3'
2nd locus specific primer 03vj: 5'-AACTTGATCAACCTCCAGAGAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11