Genotyping and primer details


Genotyping and primer details for 937G03 - At2g37480
Line ID 937G03
Locus At2g37480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15738776 of line 937G03 Chr2:15738776
   
Insertion allele amplicon 8474 - 66bk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 66bk: 5'-ACTTCCTCAGTGACAAGCCATACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15739319
   
wt amplicon (over InsPos) 01cg - 66bk
wt amplicon start Chr2:15738352
wt amplicon end Chr2:15739319
wt amplicon size 968 bp
Locus specific primer 66bk: 5'-ACTTCCTCAGTGACAAGCCATACT-3'
2nd locus specific primer 01cg: 5'-GATCTTTGGTGCTTTAACTTCTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11