Genotyping and primer details


Genotyping and primer details for 955B02 - At2g41870
Line ID 955B02
Locus At2g41870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17471355 of line 955B02 Chr2:17471355
   
Insertion allele amplicon 8474 - 29gz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 29gz: 5'-TGAGCTATGGGATTAAGTATTGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17471821
   
wt amplicon (over InsPos) 04ig - 29gz
wt amplicon start Chr2:17470779
wt amplicon end Chr2:17471821
wt amplicon size 1043 bp
Locus specific primer 29gz: 5'-TGAGCTATGGGATTAAGTATTGGG-3'
2nd locus specific primer 04ig: 5'-ACTTTTGAATCACAGTGAGAGGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11