Genotyping and primer details


Genotyping and primer details for 955D10 - At3g19760
Line ID 955D10
Locus At3g19760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6863773 of line 955D10 Chr3:6863773
   
Insertion allele amplicon 3144 - 30ah
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 30ah: 5'-ATCCAGCTAAAGGAACAGAAAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6864124
   
Insertion allele amplicon 8474 - AC88
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AC88: 5'-TTAGGAACGAGATAGGGTGAAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6863405
   
wt amplicon (over InsPos) 30ah - AC88
wt amplicon start Chr3:6863405
wt amplicon end Chr3:6864124
wt amplicon size 720 bp
Locus specific primer AC88: 5'-TTAGGAACGAGATAGGGTGAAGAA-3'
2nd locus specific primer 30ah: 5'-ATCCAGCTAAAGGAACAGAAAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11