Genotyping and primer details


Genotyping and primer details for SALK_002536 - At5g66940
Line ID SALK_002536
Locus At5g66940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26728506 of line SALK_002536 Chr5:26728506
   
Insertion allele amplicon LBb3 - 74sm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 74sm: 5'-TGGAGAGATTAGAGAATCGTGGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26728955
   
wt amplicon (over InsPos) 07tl - 74sm
wt amplicon start Chr5:26728077
wt amplicon end Chr5:26728955
wt amplicon size 879 bp
Locus specific primer 74sm: 5'-TGGAGAGATTAGAGAATCGTGGTT-3'
2nd locus specific primer 07tl: 5'-GTAGTAGTTGCAGCATCAGTAGCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11