Genotyping and primer details


Genotyping and primer details for SALK_003186 - At5g47140
Line ID SALK_003186
Locus At5g47140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19145887 of line SALK_003186 Chr5:19145887
   
Insertion allele amplicon LBb3 - 16ux
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 16ux: 5'-TGTAGGGTCCTTTTGATCACTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19145296
   
wt amplicon (over InsPos) 56vl - 16ux
wt amplicon start Chr5:19145296
wt amplicon end Chr5:19146266
wt amplicon size 971 bp
Locus specific primer 16ux: 5'-TGTAGGGTCCTTTTGATCACTCTT-3'
2nd locus specific primer 56vl: 5'-GCTCCTGCAACAACAACATTATTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11