Genotyping and primer details


Genotyping and primer details for SALK_003331 - At3g26932
Line ID SALK_003331
Locus At3g26932 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:9930877 of line SALK_003331 Chr3:9930877
   
Insertion allele amplicon LBb3 - 42us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 42us: 5'-AAAGACACAACCTTTAATCATGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:9930411
   
wt amplicon (over InsPos) 31vx - 42us
wt amplicon start Chr3:9930411
wt amplicon end Chr3:9931523
wt amplicon size 1113 bp
Locus specific primer 42us: 5'-AAAGACACAACCTTTAATCATGGG-3'
2nd locus specific primer 31vx: 5'-TCTTAGTGCTCTCTCTTCCAAAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11