Genotyping and primer details


Genotyping and primer details for SALK_004777c - At3g03660
Line ID SALK_004777c
Locus At3g03660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:891489 of line SALK_004777c Chr3:891489
   
Insertion allele amplicon LBb3 - 75sm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 75sm: 5'-CCGTTATAGCCCCTGTATTACAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:890900
   
wt amplicon (over InsPos) 06tl - 75sm
wt amplicon start Chr3:890900
wt amplicon end Chr3:891845
wt amplicon size 946 bp
Locus specific primer 75sm: 5'-CCGTTATAGCCCCTGTATTACAAA-3'
2nd locus specific primer 06tl: 5'-CACCAACACTTGTGACCAGACTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11