Genotyping and primer details


Genotyping and primer details for SALK_005045 - At3g45630
Line ID SALK_005045
Locus At3g45630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:16749296 of line SALK_005045 Chr3:16749296
   
Insertion allele amplicon LBb3 - 07tw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 07tw: 5'-GAGTGATCATAGGGCCAGTACTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:16749951
   
wt amplicon (over InsPos) 16ue - 07tw
wt amplicon start Chr3:16748849
wt amplicon end Chr3:16749951
wt amplicon size 1103 bp
Locus specific primer 07tw: 5'-GAGTGATCATAGGGCCAGTACTCT-3'
2nd locus specific primer 16ue: 5'-GTTCATTCCTTAGAAGCTGGGTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11