Genotyping and primer details


Genotyping and primer details for SALK_005080c - At3g22820
Line ID SALK_005080c
Locus At3g22820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:8074117 of line SALK_005080c Chr3:8074117
   
Insertion allele amplicon LBb3 - 76sm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 76sm: 5'-ATTGCCTCAAAGCTAACCAGTAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8073314
   
wt amplicon (over InsPos) 20tl - 76sm
wt amplicon start Chr3:8073314
wt amplicon end Chr3:8074421
wt amplicon size 1108 bp
Locus specific primer 76sm: 5'-ATTGCCTCAAAGCTAACCAGTAAA-3'
2nd locus specific primer 20tl: 5'-TACGGCTATGACATCTTTATTCCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11