Genotyping and primer details


Genotyping and primer details for SALK_005722c - At2g41010
Line ID SALK_005722c
Locus At2g41010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17114591 of line SALK_005722c Chr2:17114591
   
Insertion allele amplicon LBb3 - 43et
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 43et: 5'-TTTCTTTTTCTTTTTGGGGCTAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17115221
   
wt amplicon (over InsPos) 08gc - 43et
wt amplicon start Chr2:17114012
wt amplicon end Chr2:17115221
wt amplicon size 1210 bp
Locus specific primer 43et: 5'-TTTCTTTTTCTTTTTGGGGCTAAT-3'
2nd locus specific primer 08gc: 5'-AGATCACATAACCTTCCACGATTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11