Genotyping and primer details


Genotyping and primer details for SALK_005743 - At2g25670
Line ID SALK_005743
Locus At2g25670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10930116 of line SALK_005743 Chr2:10930116
   
Insertion allele amplicon LBb3 - 77sm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 77sm: 5'-TCTGTCGAGACTTCTAGGTGTGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10930697
   
wt amplicon (over InsPos) 01tp - 77sm
wt amplicon start Chr2:10929739
wt amplicon end Chr2:10930697
wt amplicon size 959 bp
Locus specific primer 77sm: 5'-TCTGTCGAGACTTCTAGGTGTGAA-3'
2nd locus specific primer 01tp: 5'-TTTCCTCTCTTTCTTGGAAAGTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11