Genotyping and primer details


Genotyping and primer details for SALK_006926c - At2g45880
Line ID SALK_006926c
Locus At2g45880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18882557 of line SALK_006926c Chr2:18882557
   
Insertion allele amplicon LBb3 - 06oz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 06oz: 5'-TTTGTGACTAACGAAACCAAACAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18882159
   
wt amplicon (over InsPos) 10px - 06oz
wt amplicon start Chr2:18882159
wt amplicon end Chr2:18882959
wt amplicon size 801 bp
Locus specific primer 06oz: 5'-TTTGTGACTAACGAAACCAAACAG-3'
2nd locus specific primer 10px: 5'-TTTTGTAACGAATGTTTTTGGTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11