Genotyping and primer details


Genotyping and primer details for SALK_007212 - At4g32610
Line ID SALK_007212
Locus At4g32610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15729493 of line SALK_007212 Chr4:15729493
   
Insertion allele amplicon LBb3 - 78sm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 78sm: 5'-TCTTGTTGACCATTCTCATCTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15728919
   
wt amplicon (over InsPos) 18tl - 78sm
wt amplicon start Chr4:15728919
wt amplicon end Chr4:15729979
wt amplicon size 1061 bp
Locus specific primer 78sm: 5'-TCTTGTTGACCATTCTCATCTTTG-3'
2nd locus specific primer 18tl: 5'-TGAAATTGTGAAACTTCGTTTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11