Genotyping and primer details


Genotyping and primer details for SALK_009272 - At3g50400
Line ID SALK_009272
Locus At3g50400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18706143 of line SALK_009272 Chr3:18706143
   
Insertion allele amplicon LBb3 - 49qz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 49qz: 5'-CGGTTAGGAATGGACATACAAGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18705515
   
wt amplicon (over InsPos) 09rz - 49qz
wt amplicon start Chr3:18705515
wt amplicon end Chr3:18706500
wt amplicon size 986 bp
Locus specific primer 49qz: 5'-CGGTTAGGAATGGACATACAAGTT-3'
2nd locus specific primer 09rz: 5'-CTAACCACTCTAAGCCACCAAAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11