Genotyping and primer details


Genotyping and primer details for SALK_010162 - At4g36610
Line ID SALK_010162
Locus At4g36610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17266907 of line SALK_010162 Chr4:17266907
   
Insertion allele amplicon LBb3 - 63sa
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 63sa: 5'-AGCATAGATTAACAACCCAACACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17267448
   
wt amplicon (over InsPos) 14sx - 63sa
wt amplicon start Chr4:17266624
wt amplicon end Chr4:17267448
wt amplicon size 825 bp
Locus specific primer 63sa: 5'-AGCATAGATTAACAACCCAACACC-3'
2nd locus specific primer 14sx: 5'-CTCGATGTAGTCTTTGAACAATCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11