Genotyping and primer details


Genotyping and primer details for SALK_010501c - At3g51730
Line ID SALK_010501c
Locus At3g51730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:19188354 of line SALK_010501c Chr3:19188354
   
Insertion allele amplicon LBb3 - 05rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 05rb: 5'-TTTCGACCGTTTTTAATCATTCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19188904
   
wt amplicon (over InsPos) 10se - 05rb
wt amplicon start Chr3:19188058
wt amplicon end Chr3:19188904
wt amplicon size 847 bp
Locus specific primer 05rb: 5'-TTTCGACCGTTTTTAATCATTCAT-3'
2nd locus specific primer 10se: 5'-TGGATATCGTTGAACTGCTTATCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11