Genotyping and primer details


Genotyping and primer details for SALK_011535c - At5g09760
Line ID SALK_011535c
Locus At5g09760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3032716 of line SALK_011535c Chr5:3032716
   
Insertion allele amplicon LBb3 - 30gz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 30gz: 5'-AAAACCCAAATTAAGCCCTGTACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3033144
   
wt amplicon (over InsPos) 04ir - 30gz
wt amplicon start Chr5:3032139
wt amplicon end Chr5:3033144
wt amplicon size 1006 bp
Locus specific primer 30gz: 5'-AAAACCCAAATTAAGCCCTGTACT-3'
2nd locus specific primer 04ir: 5'-ACGAAAAAGGACAGTCACAAAACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11