Genotyping and primer details


Genotyping and primer details for SALK_012200c - At1g78450
Line ID SALK_012200c
Locus At1g78450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29517191 of line SALK_012200c Chr1:29517191
   
Insertion allele amplicon R204 - SC08
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer SC08: 5'-ATCTTCGAGAAGAATGGTACGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29516188
   
wt amplicon (over InsPos) 14lt - SC08
wt amplicon start Chr1:29516188
wt amplicon end Chr1:29517516
wt amplicon size 1329 bp
Locus specific primer SC08: 5'-ATCTTCGAGAAGAATGGTACGAAA-3'
2nd locus specific primer 14lt: 5'-AGTCTCTCAGAACTTGAGCACCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11