Genotyping and primer details


Genotyping and primer details for SALK_013567c - At1g13900
Line ID SALK_013567c
Locus At1g13900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4753601 of line SALK_013567c Chr1:4753601
   
Insertion allele amplicon LBb3 - BI56
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer BI56: 5'-CCTTCCACAAAATACCATTGTTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4752852
   
wt amplicon (over InsPos) BI57 - BI56
wt amplicon start Chr1:4752852
wt amplicon end Chr1:4753836
wt amplicon size 985 bp
Locus specific primer BI56: 5'-CCTTCCACAAAATACCATTGTTCT-3'
2nd locus specific primer BI57: 5'-TACAGGTGAGTTTGGTTACACTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11