Genotyping and primer details


Genotyping and primer details for SALK_013618c - At5g15790
Line ID SALK_013618c
Locus At5g15790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5150789 of line SALK_013618c Chr5:5150789
   
Insertion allele amplicon LBb3 - 34wm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 34wm: 5'-TGTTGTTTTCTCTCAAGAGGGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5151150
   
wt amplicon (over InsPos) 30xa - 34wm
wt amplicon start Chr5:5150246
wt amplicon end Chr5:5151150
wt amplicon size 905 bp
Locus specific primer 34wm: 5'-TGTTGTTTTCTCTCAAGAGGGTTT-3'
2nd locus specific primer 30xa: 5'-CTTAAGAAGTGATGCTGATGTGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11